DSpace JSPUI
DSpace preserves and enables easy and open access to all types of digital content including text, images, moving images, mpegs and data sets
Learn More
Please use this identifier to cite or link to this item:
http://41.89.96.81:8080/xmlui/handle/123456789/2755Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Mbuthia, Peris Wanjiru | - |
| dc.date.issued | 2020-11 | - |
| dc.date.accessioned | 2021-08-23T07:46:32Z | - |
| dc.date.available | 2021-08-23T07:46:32Z | - |
| dc.identifier.uri | http://41.89.96.81:8080/xmlui/handle/123456789/2755 | - |
| dc.description.abstract | Naturalinfectionswithsoiltransmittedhelminthicnematodesoccurinnon-humanpri mates(NHPs)andhavethepotentialtocrossprimate speciesboundariesandcausediseasesofsignificantpublichealthconcern.Consequently,theidentificationandmolecularcharacterisa-tionofwormsinfectingNHPslivinginproximitytohumansiskeytosurveillanceandcontrolofzoonoticnematodesoriginatingfromwildlife.DespitethepresenceofNHPsinmosturbancentresinKenya,thereislittleinformationongeneticcharacterisationofcirculatingsoiltrans-mittedparasiticnematodesandtheroleofNHPsasréservoirsinurbansettings.Therefore,acrosssectionalsurveywasundertaken,andpolymerasechainreactioncoupledwithhigh-res-olutionmelting(PCR-HRM)analysisandmicroscopyappliedfordetectionofparasiticnema-todesinfectingfree-rangingcommonNHPsfoundwithinselectedurbancentresinKenyanamelyMombasa,Kisumu,KakamegaandMurang’a.Eighty-sixfaecalsamplesfromChlorocebusaethiops(Africangreenmonkey,AGM,n=41),Papioanubis(Olivebaboon,n=30),Cercopithecusmitis(bluemonkey,n=5)andCercopithecusascanius(red-tailedmon-key,n=10)werecollectedbyrectalswabbing.CombinationofparasitologicalidentificationandPCR-HRMdetectionofhelminthicnematoderevealedarichdiversityofhelminthsinclud-ingOesophagostomumstephanostomumandOesophagostomumbifurcum.PCR-HRManaly-sisofITS 2rDNAgenegeneratedtwodistinctprofilescorrespondingtoO.stephanostomumandO.bifurcum,andtheresultswasfurtherconfirmedbysequencing.TwoAGMswerein-fectedwithO.stephanostomumandatotalof11NHPswereco-infectedwithO.stephanostomumandO.bifurcum.Phylogenyanalysisoftheparasites’sequencesshowedO.stephanostomumandO.bifurcumclusteredwithreferencehumanisolatessuggestingclosegeneticsimilarities.Overall,thisstudydemonstratedthesuitabilityofPCR-HRMasanon-subjectivemethodforrapiddetectionanddifferentiationofnodularwormsbygenerationofdistinctmeltcurvesforeachwormspecies.Toourknowledge,wereport,forthefirsttime,theco-infectionofnodularwormsinNHPs,whichfurtherinformsontheepidemiologyofthesezoonoticnematodesinKenyaurbancentres.Finally,resultsconfirmingthatdifferentspeciesofNHPsharbourpotentiallyzoonoticnematodeswithinurbanenvironswillinformonbroaderhelminthcontrolstrategiesinendemictropicalcountrieslikeKenya. | en_US |
| dc.description.sponsorship | Consortium for National Health Re-search (CNHR) grant, Kenya (Project number RCDG-041/2012) Global Enhancement Fund grant, USA (2017) | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Egerton University | en_US |
| dc.subject | Molecular Detection | en_US |
| dc.title | Molecular Detection of Zoonotic Helminths Infection Non-Human Primates in Kenyan Urban Centres | en_US |
| dc.type | Thesis | en_US |
| Appears in Collections: | Faculty of Science | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Molecular Detection of Zoonotic Helminths Infection Non-Human Primates in Kenyan Urban Centres.pdf | 2.23 MB | Adobe PDF |  View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.