Molecular Detection of Zoonotic Helminths Infection Non-Human Primates in Kenyan Urban Centres

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dc.contributor.authorMbuthia, Peris Wanjiru
dc.date.accessioned2021-08-23T07:46:32Z
dc.date.accessioned2024-09-16T11:24:51Z
dc.date.available2021-08-23T07:46:32Z
dc.date.available2024-09-16T11:24:51Z
dc.date.issued2020-11
dc.description.abstractNaturalinfectionswithsoiltransmittedhelminthicnematodesoccurinnon-humanpri mates(NHPs)andhavethepotentialtocrossprimate speciesboundariesandcausediseasesofsignificantpublichealthconcern.Consequently,theidentificationandmolecularcharacterisa-tionofwormsinfectingNHPslivinginproximitytohumansiskeytosurveillanceandcontrolofzoonoticnematodesoriginatingfromwildlife.DespitethepresenceofNHPsinmosturbancentresinKenya,thereislittleinformationongeneticcharacterisationofcirculatingsoiltrans-mittedparasiticnematodesandtheroleofNHPsasréservoirsinurbansettings.Therefore,acrosssectionalsurveywasundertaken,andpolymerasechainreactioncoupledwithhigh-res-olutionmelting(PCR-HRM)analysisandmicroscopyappliedfordetectionofparasiticnema-todesinfectingfree-rangingcommonNHPsfoundwithinselectedurbancentresinKenyanamelyMombasa,Kisumu,KakamegaandMurang’a.Eighty-sixfaecalsamplesfromChlorocebusaethiops(Africangreenmonkey,AGM,n=41),Papioanubis(Olivebaboon,n=30),Cercopithecusmitis(bluemonkey,n=5)andCercopithecusascanius(red-tailedmon-key,n=10)werecollectedbyrectalswabbing.CombinationofparasitologicalidentificationandPCR-HRMdetectionofhelminthicnematoderevealedarichdiversityofhelminthsinclud-ingOesophagostomumstephanostomumandOesophagostomumbifurcum.PCR-HRManaly-sisofITS 2rDNAgenegeneratedtwodistinctprofilescorrespondingtoO.stephanostomumandO.bifurcum,andtheresultswasfurtherconfirmedbysequencing.TwoAGMswerein-fectedwithO.stephanostomumandatotalof11NHPswereco-infectedwithO.stephanostomumandO.bifurcum.Phylogenyanalysisoftheparasites’sequencesshowedO.stephanostomumandO.bifurcumclusteredwithreferencehumanisolatessuggestingclosegeneticsimilarities.Overall,thisstudydemonstratedthesuitabilityofPCR-HRMasanon-subjectivemethodforrapiddetectionanddifferentiationofnodularwormsbygenerationofdistinctmeltcurvesforeachwormspecies.Toourknowledge,wereport,forthefirsttime,theco-infectionofnodularwormsinNHPs,whichfurtherinformsontheepidemiologyofthesezoonoticnematodesinKenyaurbancentres.Finally,resultsconfirmingthatdifferentspeciesofNHPsharbourpotentiallyzoonoticnematodeswithinurbanenvironswillinformonbroaderhelminthcontrolstrategiesinendemictropicalcountrieslikeKenya.en_US
dc.description.sponsorshipConsortium for National Health Re-search (CNHR) grant, Kenya (Project number RCDG-041/2012) Global Enhancement Fund grant, USA (2017)en_US
dc.identifier.urihttp://172.16.31.117:4000/handle/123456789/2755
dc.language.isoenen_US
dc.publisherEgerton Universityen_US
dc.subjectMolecular Detectionen_US
dc.titleMolecular Detection of Zoonotic Helminths Infection Non-Human Primates in Kenyan Urban Centresen_US
dc.typeThesisen_US

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