DSpace Repository

In vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson)

Show simple item record

dc.contributor.author Mulwa, Richard M. S.
dc.contributor.author Bhalla, Prem L.
dc.date.issued 2006-07
dc.date.accessioned 2021-05-21T07:27:39Z
dc.date.available 2021-05-21T07:27:39Z
dc.identifier.uri http://41.89.96.81:8080/xmlui/handle/123456789/2550
dc.description.abstract The macadamia tree, an Australian native, is highly valued for its nuts. Macadamia improvement pro- grams so far have relied on conventional breeding and selec- tion. The production of improved cultivars required to meet future demands could be accelerated by the application of modern biotechnological techniques, but this requires an efficient and reproducible regeneration system that has not yet been established for macadamia. We report here shoot regeneration from immature cotyledon explants of macadamia. Adventitious buds were induced on the cotyle- don explants from fruits collected at 140 and 190 days af- ter full bloom (DAFB) on MS medium supplemented with either 10 or 15 µM TDZ. The addition of 2% coconut milk (CM) to 10 µM TDZ containing media resulted in enhanced adventitious bud induction from 190 DAFB ex- plants. Further shoot development from the induced buds was depressed in media containing TDZ + CM; the addi- tion of 0.001 µM IAA to this combination doubled shoot development, from 1.9–3.9 shoots per explant. The trans- fer of bud clumps to media supplemented with 8.8 µM BA alone or in combination with either 0.14 µM GA3 or 0.001 µM IAA significantly increased shoot production from the previously induced explants by 1.5–2 times of that observed in TDZ + CM medium. en_US
dc.language.iso en en_US
dc.publisher Egerton University en_US
dc.subject Somatic embryogenesis en_US
dc.subject Histology en_US
dc.title In vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson) en_US
dc.type Other en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account